Allergology: Diagnostic Methods | Treatise on Clinical Immunology and High Complexity Allergological Diagnosis

Allergology and Immunology · Clinical Specialties

How it begins

An exhaustive treatise on the biophysical, molecular, cellular and clinical provocation foundations aimed at confirming allergic pathology. It includes the molecular dissection of component diagnosis, standardized controlled provocation protocols and the critical analysis of in vitro cellular activation techniques.

What it covers

1. Immunological Basis of Allergological Diagnosis
2. · Immunological Mechanisms of Sensitization and Hypersensitivity Type I
3. In Vivo Skin Tests: Prick Test
4. · Standardized Prick Test Technique
5. Intradermal Tests (IDR)
6. · Specific Clinical Indications of the IDR
7. Patch Tests in Contact Dermatitis
8. · Pathophysiology of Allergic Contact Dermatitis
9. In vitro diagnosis: Quantification of Total and Specific Serum IgE (sIgE)
10. · Total Serum IgE: Clinical Significance and Limitations
11. Molecular Component Diagnosis (CRD - Component-Resolved Diagnosis)
12. · Allergenic Protein Families and Immunological Classification
13. Basophil Activation Test (TAB / BAT)
14. · Physiology of Membrane Labeling in Basophils
15. Mediator Release Tests (Histamine, Leukotrienes, Tryptase)
16. · Mastocyte Tryptase: Kinetics and Diagnosis of Anaphylaxis
17. Ocular, Nasal and Bronchial Diagnosis: Mucosal Provocation Tests
18. · Nasal Provocation Test (NPT)
19. Oral Food Provocation Tests (PPO / OFC)
20. · PPO methodologies: Open, Single Blind and Double Blind
21. Provocation Tests with Medications (PPM / DPT)
22. · General Principles and Absolute Contraindications of PPM
23. Diagnosis of Hymenoptera Venom Allergy
24. · Venom Immunology and Key Molecular Components
25. Diagnosis of Occupational and Industrial Allergy
26. · High and Low Molecular Weight Agents
27. Latex Allergy and Cross Reactivity Syndromes
28. · Molecular Components of Hevea brasiliensis Latex
29. Diagnosis of Non-IgE-Mediated Reactions
30. · Dietary Protein Induced Enterocolitis Syndrome (FPIES)
31. Diagnostic Methods in Urticaria and Angioedema
32. · Differentiation of Bradykinin-Mediated Angioedema vs. Histaminergic
33. Diagnosis of Fungal Allergies and Allergic Bronchopulmonary Aspergillosis
34. · Main Anemophilous Fungi and Their Recombinant Components
35. New Technologies: Microarrays, Nanotechnology and Multiplex
36. · The ISAC and ALEX2 Platforms
37. Common Errors, False Positives/Negatives and Methods Without Scientific Evidence
38. · False Positives and Negatives in Conventional Diagnostic Methods
39. Clinical Decision Algorithms and Diagnostic Integration
40. · The Golden Triangle of Diagnostic Integration