Molecular Diagnosis and Immunological Serology — Medical Microbiology

Microbiology · Basic Sciences

How it begins

Polymerase chain reaction (PCR, qPCR, Ct threshold cycle), multiplex syndromic panels, clinical metagenomics, antibody kinetics and IgG avidity testing.

What it covers

1. 1. Fundamentals of the Polymerase Chain Reaction (PCR)
2. 2. Real-Time PCR (qPCR), Viral Load Quantification and Cycle Threshold (Ct)
3. 3. Multiplex Syndromic Panels in Emergency and Critical Care
4. 4. Critical Clinical Interpretation: Detectable DNA vs Active Infection
5. 5. Immunological Serology: IgM and IgG Kinetics and the Avidity Test
6. 6. Serological Methods: ELISA, Western Blot and POC Rapid Tests
7. 7. Which Test at Which Time
8. 8. Syndromic Panels, Ct and Sequencing: Using Them Well
9. 9. Reasoned Clinical Cases
10. 10. Common Mistakes and Exam Pearls
11. 11. References and Sources for Further Reading