Clinical Biochemistry — Transcriptional Machinery and Regulation of Gene Expression

Biochemistry · Basic Sciences

How it begins

Comprehensive scientific compendium on the eukaryotic transcription apparatus, chromatin remodeling complexes, regulation by cis and trans elements, the histone code and DNA methylation. It includes a rigorous clinical analysis of pathologies derived from dysfunctions in gene expression.

What it covers

1. Eukaryotic Genome Architecture and Chromatin Structure
2. · Hierarchical Levels of Chromatin Condensation
3. · Functional Dichotomy: Euchromatin vs Heterochromatin
4. Eukaryotic RNA Polymerases
5. · Detailed Structure of RNA Polymerase II and the CTD Domain
6. The Preinitiation Complex (PIC) and Basal Assembly
7. Cis Regulatory Elements and Trans Factors
8. · 1. Cis Action Elements
9. · 2. Factors in Trans: Structure and Reasons for DNA Binding
10. The Mediator Complex and Transcriptional Coactivators
11. · Transcriptional Coactivators: p300/PBC
12. The Transcription Cycle: Phases and Dynamic Regulation
13. Co-transcriptional processing of RNA
14. Epigenetic Regulation I: The Histone Code
15. Epigenetic Regulation II: DNA Methylation
16. · Biochemical Mechanisms of Methylation-Mediated Gene Silencing
17. · The Active DNA Demethylation Process
18. ATP-Dependent Chromatin Remodeling
19. Post-transcriptional regulation by non-coding RNA
20. · MicroRNAs (miRNAs): Biogenesis and Functional Mechanism
21. · Long Non-Coding RNAs (lncRNAs): Regulators of Epigenetic Architecture
22. Methods for the Study of Gene Regulation
23. Clinical Correlations and Molecular Physiopathology
24. · Clinical Synthesis: Gene Expression as a Modern Pharmacological Target